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At Neopharm, we understand that the nuances matter. Our services offer high sensitivity and specificity, ensuring that you can detect even low-abundance proteins or subtle changes in expression levels. Whether you're exploring disease markers, studying signaling pathways, or assessing therapeutic efficacy, our precise techniques guide you to meaningful conclusions.
Neopharm's SDS-PAGE and Western blotting services offer reliable protein separation based on size and charge as well as a specific detection of a target protein. By employing this technique, we enable you to visualize and distinguish proteins within complex mixtures, providing valuable insights into their composition and relative abundance. Our expertise in SDS-PAGE and Western blotting ensures that you can analyze protein samples with precision and clarity.
Quality control is paramount in the biotech industry. Neopharm's SDS-PAGE services play a crucial role in assessing protein purity and confirming the absence of contaminants. By comparing the migration patterns of your samples to known standards, we can detect and determine the molecular weights of impurities, protein variants, or degradation products. Our detailed analysis enables you to ensure the integrity and purity of your protein samples, maintaining the highest quality standards in your biotech research and development.
Quantification of proteins is essential for many biotech applications. Neopharm's SDS-PAGE services can be used for semi-quantitative analysis of protein abundance. By comparing the intensity of protein bands to known standards or internal controls, we provide relative protein quantification, allowing you to assess changes in protein levels across different samples or experimental conditions. Our expertise in protein quantification ensures reliable and reproducible results for your biotech projects.
Neopharm offers band excision services as part of our SDS-PAGE offering. If you need to isolate specific protein bands for downstream applications, such as mass spectrometry or protein sequencing, our skilled scientists can precisely excise the bands of interest. We ensure minimal contamination and provide purified protein samples for further analysis, accelerating your biotech research and enabling deeper insights into protein function and structure.
SDS-PAGE, or Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, is a widely used technique for separating proteins based on their molecular weight. In SDS-PAGE, proteins are denatured and coated with SDS, a detergent that imparts a negative charge. The SDS-coated proteins are then subjected to electrophoresis through a porous polyacrylamide gel matrix. The proteins migrate based on their size, with smaller proteins moving faster through the gel. This separation allows for the visualization and analysis of protein bands.
SDS-PAGE can be used to analyze a wide range of protein samples, including purified proteins, cell lysates, tissue extracts, and biological fluids. Neopharm's SDS-PAGE services accommodate various sample types, ensuring that you can assess the protein composition and purity of your specific samples in the biotech market.
SDS-PAGE offers several advantages for protein analysis. It provides high-resolution separation, allowing for the detection of protein variants and impurities. SDS-PAGE is relatively simple, cost-effective, and compatible with a wide range of sample types. Additionally, it can be used for both qualitative and semi-quantitative analysis, making it a versatile tool in the biotech industry.
With Neopharm's SDS-PAGE and Western Blotting services, you're not just getting services; you're obtaining precision, expertise, and a partner committed to driving your discoveries forward.Contact us